Journal: Immunity, Inflammation and Disease

Article Title: Broad Th2 neutralization and anti‐inflammatory action of pentosan polysulfate sodium in experimental allergic rhinitis

doi: 10.1002/iid3.164

Figure Lengend Snippet: Molecular interactions and binding of PPS and heparin to Th2 cytokines as revealed by surface plasmon resonance (SPR) methodology. Data are shown for representative titration experiments showing the capacity of soluble heparin (dashed lines) and PPS (solid lines) to bind (A) IL‐4, (B) IL‐5, and (C) IL‐13 and prevent attachment to a cytokine‐binding detector chip. The experiments were performed on the BIAcore 2000 platform and with a heparinised CM4 flow cell detector .

Article Snippet: Biotinylated heparin was immobilized on CM‐4 sensor chips pre‐coated with streptavidin, and real‐time biomolecular interaction analyses were performed with a BIAcore 2000 SPR biosensor , .

Techniques: Binding Assay, SPR Assay, Titration